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Furthermore, we hypothesized which the size of your TAL binding sequences really should correlate Together with the diploma of similarity concerning the Flp binding aspects from the FRT-like sequence Which of FRT: the weaker the similarity, the for a longer period the TAL binding sequence.

Considering that equally the Flp plus the TAL modules of the former type have one of a kind focus on specificity, the hybrid recombinases of this kind are anticipated to become one of the most unique genome modifying tools. The plain limitation of such Flp-TAL recombinases is a substantial energy which is needed to evolve remarkably distinct Flp modules.

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